uls ® microrna labeling kit (Kreatech Diagnostics)
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Uls ® Microrna Labeling Kit, supplied by Kreatech Diagnostics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/uls+labeling/mirna+ulstm+labeling+kit/pmc11432929-185-18-23
Average 90 stars, based on 1 article reviews
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Microarray:Article Title: DMH Ppp1r17 neurons regulate aging and lifespan in mice through hypothalamic-adipose inter-tissue communication. Article Snippet: Approximately 500-1000 cells were pooled from each hypothalamic nucleus from individual mice, and RNA was isolated using the proteinase K/acid phenol method as described previously.69 The quality of total RNAwas determined by Agilent 2100 Bioanalyzer (Agilent Technologies), and 2-3 samples from each hypothalamic nucleus were used for microarray analysis. .. The detailed procedure for microarray analysis was described previously.70 Briefly, total RNA was amplified using the WTA2 kit (Sigma-Aldrich) according to the manufacturer’s protocol. cDNAs were chemically labeled with Amplification:Article Title: DMH Ppp1r17 neurons regulate aging and lifespan in mice through hypothalamic-adipose inter-tissue communication. Article Snippet: Approximately 500-1000 cells were pooled from each hypothalamic nucleus from individual mice, and RNA was isolated using the proteinase K/acid phenol method as described previously.69 The quality of total RNAwas determined by Agilent 2100 Bioanalyzer (Agilent Technologies), and 2-3 samples from each hypothalamic nucleus were used for microarray analysis. .. The detailed procedure for microarray analysis was described previously.70 Briefly, total RNA was amplified using the WTA2 kit (Sigma-Aldrich) according to the manufacturer’s protocol. cDNAs were chemically labeled with Labeling:Article Title: DMH Ppp1r17 neurons regulate aging and lifespan in mice through hypothalamic-adipose inter-tissue communication. Article Snippet: Approximately 500-1000 cells were pooled from each hypothalamic nucleus from individual mice, and RNA was isolated using the proteinase K/acid phenol method as described previously.69 The quality of total RNAwas determined by Agilent 2100 Bioanalyzer (Agilent Technologies), and 2-3 samples from each hypothalamic nucleus were used for microarray analysis. .. The detailed procedure for microarray analysis was described previously.70 Briefly, total RNA was amplified using the WTA2 kit (Sigma-Aldrich) according to the manufacturer’s protocol. cDNAs were chemically labeled with Article Title: Regulation of RNase E during the UV stress response in the cyanobacterium Synechocystis sp. PCC 6803 Article Snippet: .. Total RNA (5 μg) was extracted from Synechocystis 6803 cells collected 1 or 2 h after UV‐C irradiation at 400 J/m 2 or following mock treatment and directly labeled with Cy5 (without cDNA synthesis) using the Article Title: Murine fecal microRNAs alter the composition of cultured gut microbiota. Article Snippet: Fecal microRNAs (miRNAs) derived from intestinal epithelial cells have been suggested to influence gut microbiota homeostasis.. The present study examined whether fecal miRNAs alter the structure of cultured gut microbiota.. Fecal bacteria isolated from murine cecal contents were cultured for 24 h under anaerobic conditions. Article Title: The Gene Expression Profile in the Synovium as a Predictor of the Clinical Response to Infliximab Treatment in Rheumatoid Arthritis Article Snippet: .. Labeling was performed with the Article Title: Regulation of RNase E during the UV stress response in the cyanobacterium <i>Synechocystis</i> sp. PCC 6803 Article Snippet: .. Total RNA (5 μg) was extracted from Synechocystis 6803 cells collected 1 or 2 h after UV‐C irradiation at 400 J/m2 or following mock treatment and directly labeled with Cy5 (without cDNA synthesis) using the Purification:Article Title: DMH Ppp1r17 neurons regulate aging and lifespan in mice through hypothalamic-adipose inter-tissue communication. Article Snippet: Approximately 500-1000 cells were pooled from each hypothalamic nucleus from individual mice, and RNA was isolated using the proteinase K/acid phenol method as described previously.69 The quality of total RNAwas determined by Agilent 2100 Bioanalyzer (Agilent Technologies), and 2-3 samples from each hypothalamic nucleus were used for microarray analysis. .. The detailed procedure for microarray analysis was described previously.70 Briefly, total RNA was amplified using the WTA2 kit (Sigma-Aldrich) according to the manufacturer’s protocol. cDNAs were chemically labeled with Polymerase Chain Reaction:Article Title: DMH Ppp1r17 neurons regulate aging and lifespan in mice through hypothalamic-adipose inter-tissue communication. Article Snippet: Approximately 500-1000 cells were pooled from each hypothalamic nucleus from individual mice, and RNA was isolated using the proteinase K/acid phenol method as described previously.69 The quality of total RNAwas determined by Agilent 2100 Bioanalyzer (Agilent Technologies), and 2-3 samples from each hypothalamic nucleus were used for microarray analysis. .. The detailed procedure for microarray analysis was described previously.70 Briefly, total RNA was amplified using the WTA2 kit (Sigma-Aldrich) according to the manufacturer’s protocol. cDNAs were chemically labeled with other:Article Title: MicroRNA Signature in an In Vitro Keratinocyte Model of Diabetic Wound Healing Article Snippet: Small RNAs (<200 nucleotides) were enriched using cutoff concentrators (Sartorius, Gottingen, Germany). miRNAs were labeled with Cy5 dye ( Article Title: Induction of Olfaction and Cancer-Related Genes in Mice Fed a High-Fat Diet as Assessed through the Mode-of-Action by Network Identification Analysis Article Snippet: For analysis, 2 μg Article Title: Circulating microRNA signatures in mice exposed to lipoteichoic acid Article Snippet: Mouse genome-wide miRNA microarray analysis was performed by Phalanx Biotech with the Mouse & Rat miRNA OneArray 1.0 (Phalanx Biotech Group, Hsinchu, Taiwan) contains a total of 2,319 probes, including 135 experimental control probes and 728 unique miRNA probes from mouse and 348 from rat (miRBase Release 12.0). Irradiation:Article Title: Regulation of RNase E during the UV stress response in the cyanobacterium Synechocystis sp. PCC 6803 Article Snippet: .. Total RNA (5 μg) was extracted from Synechocystis 6803 cells collected 1 or 2 h after UV‐C irradiation at 400 J/m 2 or following mock treatment and directly labeled with Cy5 (without cDNA synthesis) using the Article Title: Regulation of RNase E during the UV stress response in the cyanobacterium <i>Synechocystis</i> sp. PCC 6803 Article Snippet: .. Total RNA (5 μg) was extracted from Synechocystis 6803 cells collected 1 or 2 h after UV‐C irradiation at 400 J/m2 or following mock treatment and directly labeled with Cy5 (without cDNA synthesis) using the cDNA Synthesis:Article Title: Regulation of RNase E during the UV stress response in the cyanobacterium Synechocystis sp. PCC 6803 Article Snippet: .. Total RNA (5 μg) was extracted from Synechocystis 6803 cells collected 1 or 2 h after UV‐C irradiation at 400 J/m 2 or following mock treatment and directly labeled with Cy5 (without cDNA synthesis) using the Article Title: Regulation of RNase E during the UV stress response in the cyanobacterium <i>Synechocystis</i> sp. PCC 6803 Article Snippet: .. Total RNA (5 μg) was extracted from Synechocystis 6803 cells collected 1 or 2 h after UV‐C irradiation at 400 J/m2 or following mock treatment and directly labeled with Cy5 (without cDNA synthesis) using the Isolation:Article Title: Murine fecal microRNAs alter the composition of cultured gut microbiota. Article Snippet: Fecal microRNAs (miRNAs) derived from intestinal epithelial cells have been suggested to influence gut microbiota homeostasis.. The present study examined whether fecal miRNAs alter the structure of cultured gut microbiota.. Fecal bacteria isolated from murine cecal contents were cultured for 24 h under anaerobic conditions. |
